Estimation of Significance of Alkb and Alka Proteins in Dna Repair in Escherichia Coli Model
نویسندگان
چکیده
The paper concerns estimation of significance of differences of mutagenesis level between the wild-type strain (wt) and its derivatives which differ in DNA repair ability, namely alkA and alkB strain, devoided AlkA glycosylase and AlkB dioxygenase activity, respectively. The strains were analyzed for their ability to repair 1,N-ethenoadenine (εA) – chloroacetaldehyde adduct to DNA. The analysis was done using classical statistical and pattern recognition methods. The obtained results confirmed that AlkB dioxygenase plays the most important role in εA repair in E. coli in the experimental modeling.
منابع مشابه
Pseudomonas putida AlkA and AlkB Proteins Comprise Different Defense Systems for the Repair of Alkylation Damage to DNA – In Vivo, In Vitro, and In Silico Studies
Alkylating agents introduce cytotoxic and/or mutagenic lesions to DNA bases leading to induction of adaptive (Ada) response, a mechanism protecting cells against deleterious effects of environmental chemicals. In Escherichia coli, the Ada response involves expression of four genes: ada, alkA, alkB, and aidB. In Pseudomonas putida, the organization of Ada regulon is different, raising questions ...
متن کاملAda response – a strategy for repair of alkylated DNA in bacteria
Alkylating agents are widespread in the environment and also occur endogenously. They can be cytotoxic or mutagenic to the cells introducing alkylated bases to DNA or RNA. All organisms have evolved multiple DNA repair mechanisms to counteract the effects of DNA alkylation: the most cytotoxic lesion, N(3)-methyladenine (3meA), is excised by AlkA glycosylase initiating base excision repair (BER)...
متن کاملBacterial DNA repair genes and their eukaryotic homologues: 3. AlkB dioxygenase and Ada methyltransferase in the direct repair of alkylated DNA.
Environmental and endogenous alkylating agents generate cytotoxic and mutagenic lesions in DNA. Exposure of prokaryotic cells to sublethal doses of DNA alkylating agents induces so called adaptive response (Ada response) involving the expression of a set of genes which allows the cells to tolerate the toxic and mutagenic action of such agents. The Ada response includes the expression of four ge...
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The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is known to trigger the adaptive response by inducing the ada-regulon - consisting of three DNA repair enzymes Ada, AlkB, AlkA and the enigmatic AidB. We have applied custom designed tiling arrays to study transcriptional changes in Escherichia coli following a MNNG challenge. Along with the expected upregulation of the adaptive r...
متن کاملThe AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein.
Upon exposure to alkylating agents, Escherichia coli increases expression of aidB along with three genes (ada, alkA, and alkB) that encode DNA repair proteins. In order to begin to identify the role of AidB in the cell, the protein was purified to homogeneity, shown to possess stoichiometric amounts of flavin adenine dinucleotide (FAD), and confirmed to have low levels of isovaleryl-coenzyme A ...
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